anti human rabbit polyclonal p bad136  (Cell Signaling Technology Inc)

Journal: Molecular and cellular endocrinology

Article Title: DUAL INHIBITION OF ERK1/2 AND AKT PATHWAYS IS REQUIRED TO SUPPRESS THE GROWTH AND SURVIVAL OF ENDOMETRIOTIC CELLS AND LESIONS

doi: 10.1016/j.mce.2018.12.011

Figure Lengend Snippet: Details of the antibody used.

Article Snippet: Anti-human rabbit polyclonal p-Bad136 , Cell Signaling , 9295 , 1:500.

Techniques: Concentration Assay

Journal: Annals of oncology : official journal of the European Society for Medical Oncology

Article Title: Akt activation in renal cell carcinoma: contribution of a decreased PTEN expression and the induction of apoptosis by an Akt inhibitor.

doi: 10.1093/annonc/mdi182

Figure Lengend Snippet: Figure 1. (A) Representative samples of western blotting for PTEN, total Akt, p-Akt, total Bad and p-Bad in renal cell carcinoma (RCC) tissue (T) and the corresponding non-neoplastic kidney tissue (N). #1–#3 are clear cell type, #39 and #40 are papillary cell type and #41 is chromophobe cell type. b-actin is used for an internal control. (B) The ratio of p-Akt to b-actin in 45 samples. The p-Akt expression in RCC increases significantly in comparison with that in the corresponding normal kidney tissue (P <0.0001). (C) PTEN expression in RCC was observed to decrease significantly more than that in kidney tissue (P <0.0001). (D) Correlation between the p-Akt expression and PTEN expression (r = 0.313; n = 45; P = 0.0360).

Article Snippet: Rabbit polyclonal antibodies against Phospho-Bad at Ser136 (p-Bad) were obtained from Cell Signaling Technology, Inc. (Beverly, MA).

Techniques: Western Blot, Control, Expressing, Comparison

Journal: Annals of oncology : official journal of the European Society for Medical Oncology

Article Title: Akt activation in renal cell carcinoma: contribution of a decreased PTEN expression and the induction of apoptosis by an Akt inhibitor.

doi: 10.1093/annonc/mdi182

Figure Lengend Snippet: Figure 2. (A) Dose-dependent growth inhibition of Akt inhibitor on four renal cell carcinoma cell lines, Caki-1, KU19-20, SW839 and Caki-2. A growth inhibition was prominent in KU19-20 and Caki-2 by the Akt inhibitor at 48 h. Bars: S.D.; *P<0.05, **P<0.01. (B) Detection of apoptosis by the TUNEL method using a flowcytometer. A prominent shift of the cells with a DNA double-strand break was observed in KU19-20 cells treated by a 20 mM Akt inhibitor. The shift was also observed in Caki-2 cells, but not in Caki-1 and SW839. The cells with log fluorescence intensity >101 were considered to be positive for apoptosis. (C) The effects of an Akt inhibitor on the expression of PTEN, Akt, p-Akt, Bad, p-Bad and Bcl-xL determined by western blotting. Note the prominent downregulation of p-Akt and p-Bad in the KU19-20 and Caki-2 cells. (D) The bands in (C) are quantified and the ratio of p-Akt to the total Akt expression and the ratio of p-Bad to the total Bad expression are shown as graphs. Bad dephosphorylation was correlated with the Akt dephos- phorylation time-dependently in KU19-20 and Caki-2 cells.

Article Snippet: Rabbit polyclonal antibodies against Phospho-Bad at Ser136 (p-Bad) were obtained from Cell Signaling Technology, Inc. (Beverly, MA).

Techniques: Inhibition, TUNEL Assay, Expressing, Western Blot, De-Phosphorylation Assay